Anti-Bcl3抗体[Ham150-3.5] (ab15046)
Key features and details
- Hamster monoclonal [Ham150-3.5] to Bcl3
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-Bcl3抗体[Ham150-3.5]
参阅全部 Bcl3 一抗 -
描述
仓鼠单克隆抗体[Ham150-3.5] to Bcl3 -
宿主
Hamster -
特异性
ab15046 recognises Bcl3 from transfected cells and shows no cross reactivity to other Bcl isoforms. -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length protein corresponding to Mouse Bcl3.
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阳性对照
- C57BI/6 human T cells and Bcl3 transgenic ms T cells. Flow Cyt: BAF3, Ramos cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
Ham150-3.5 -
同种型
IgG -
研究领域
相关产品
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab15046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa).
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Flow Cyt |
Use at an assay dependent concentration.
1 µg/test (100µl) |
说明 |
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WB
Use a concentration of 2 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 50 kDa). |
Flow Cyt
Use at an assay dependent concentration. 1 µg/test (100µl) |
靶标
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功能
Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation. -
疾病相关
Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions. -
序列相似性
Contains 7 ANK repeats. -
翻译后修饰
Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
Activated by phosphorylation. -
细胞定位
Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. - Information by UniProt
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数据库链接
- Entrez Gene: 602 Human
- Entrez Gene: 12051 Mouse
- Omim: 109560 Human
- SwissProt: P20749 Human
- SwissProt: Q9Z2F6 Mouse
- Unigene: 31210 Human
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别名
- B cell CLL/lymphoma 3 antibody
- B cell leukemia/lymphoma 3 antibody
- B cell lymphoma 3 encoded protein antibody
see all
图片
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Flow cytometric analysis of 2% paraformaldehyde-fixed Ramos cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Flow cytometric analysis of 2% paraformaldehyde-fixed BAF3cells staining BCL-3 with ab15046 at 1µg/mL concentration and Dylight 488-conjugated secondary antibody (green), and isotype control (blue).
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Anti-Bcl3 antibody [Ham150-3.5] (ab15046) at 2 µg/ml
Predicted band size: 50 kDa
Western blot of Bcl3 in transgenic mouse T cells using Ab15046.
数据表及文件
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Datasheet download
文献 (0)
ab15046 尚未被引用在任何文献中。