概述

  • 产品名称
  • 描述
    兔多克隆抗体to Bcl2L2
  • 经测试应用
    适用于: IHC-FoFr, ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    This antibody was developed against a synthetic peptide corresponding within amino acid residues 1-100 of human Bcl2L2.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab38629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr Use at an assay dependent concentration. PubMed: 21289171
ELISA 1/1000.
WB 1/100 - 1/500. Predicted molecular weight: 21 kDa.
IHC-P 1/50 - 1/100.

靶标

  • 功能
    Promotes cell survival. Blocks dexamethasone-induced apoptosis. Mediates survival of postmitotic Sertoli cells by suppressing death-promoting activity of BAX.
  • 组织特异性
    Expressed (at protein level) in a wide range of tissues with highest levels in brain, spinal cord, testis, pancreas, heart, spleen and mammary glands. Moderate levels found in thymus, ovary and small intestine. Not detected in salivary gland, muscle or liver. Also expressed in cell lines of myeloid, fibroblast and epithelial origin. Not detected in most lymphoid cell lines.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    The BH4 motif seems to be involved in the anti-apoptotic function.
    The BH1 and BH2 motifs form a hydrophobic groove which acts as a docking site for the BH3 domain of some pro-apoptotic proteins. The C-terminal residues of BCL2L2 fold into the BH3-binding cleft and modulate pro-survival activity by regulating ligand access. When BH3 domain-containing proteins bind, they displace the C-terminus, allowing its insertion into the membrane and neutralizing the pro-survival activity of BCL2L2.
  • 细胞定位
    Mitochondrion membrane. Loosely associated with the mitochondrial membrane in healthy cells. During apoptosis, tightly bound to the membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator BCL W antibody
    • Apoptosis regulator Bcl-W antibody
    • B2CL2_HUMAN antibody
    • BCL 2 Like 2 antibody
    • Bcl 2 like 2 protein antibody
    • Bcl 2L2 antibody
    • BCL W antibody
    • Bcl-2-like protein 2 antibody
    • Bcl2 L2 antibody
    • BCL2 like 2 antibody
    • BCL2 like 2 protein antibody
    • Bcl2-L-2 antibody
    • Bcl2l2 antibody
    • BCLW antibody
    • KIAA0271 antibody
    • PPP1R51 antibody
    • Protein phosphatase 1 regulatory subunit 51 antibody
    see all

Anti-Bcl2L2 antibody 图像

  • Anti-Bcl2L2 antibody (ab38629) + HL-60 cell lysate

    Predicted band size : 21 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Anti-Bcl2L2 antibody (ab38629)参考文献

This product has been referenced in:
  • Abbaspour Babaei M  et al. Apoptotic induction and inhibition of NF-?B signaling pathway in human prostatic cancer PC3 cells by natural compound 2,2'-oxybis (4-allyl-1-methoxybenzene), biseugenol B, from Litsea costalis: an in vitro study. Onco Targets Ther 10:277-294 (2017). Read more (PubMed: 28138251) »
  • Ibrahim MY  et al. a-Mangostin from Cratoxylum arborescens demonstrates apoptogenesis in MCF-7 with regulation of NF-?B and Hsp70 protein modulation in vitro, and tumor reduction in vivo. Drug Des Devel Ther 8:1629-47 (2014). WB ; Human . Read more (PubMed: 25302018) »

See all 4 Publications for this product

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