RabMAb

Anti-BCL2L12抗体[EPR5201] (ab108346)

概述

  • 产品名称Anti-BCL2L12抗体[EPR5201]
    参阅全部 BCL2L12 一抗
  • 描述
    兔单克隆抗体[EPR5201] to BCL2L12
  • 经测试应用适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human BCL2L12.

  • 阳性对照
    • U87-MG, HT-1376, MCF7, and PC-3 cell lysates; Human breast carcinoma tissue IF/ICC: HeLa cell line.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 组织特异性Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.
  • 序列相似性Belongs to the Bcl-2 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2L12_HUMAN antibody
    • BCL 2 like 12 (proline rich) antibody
    • BCL 2 like 12 antibody
    • BCL 2 like 12 isoform 1 antibody
    • Bcl 2 like 12 protein antibody
    • Bcl 2 related proline rich protein antibody
    • Bcl-2-like protein 12 antibody
    • Bcl-2-related proline-rich protein antibody
    • BCL2 like 12 (proline rich) antibody
    • BCL2 like 12 antibody
    • BCL2 like 12 isoform 1 antibody
    • Bcl2 like 12 protein antibody
    • Bcl2 related proline rich protein antibody
    • Bcl2-L-12 antibody
    • BCL2L12 antibody
    • BPR antibody
    • MGC120313 antibody
    • MGC120314 antibody
    • MGC120315 antibody
    see all

Anti-BCL2L12 antibody [EPR5201] 图像

  • ICC/IF image of ab108346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108346, 1/100) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Overlay histogram showing HeLa cells stained with ab108346 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108346, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • All lanes : Anti-BCL2L12 antibody [EPR5201] (ab108346) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HT-1376 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of BCL2L12 in paraffin embedded Human breast carcinoma tissue, using ab108346 at a dilution of 1/500.

Anti-BCL2L12 antibody [EPR5201] (ab108346)参考文献

ab108346 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Permeabilization Yes - 0.5% Triton X100 in PBS
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

提交于 Jul 10 2014

You're welcome!

I wish you the best of luck with your research in the future. Please do not hesitate to contact me if I can be of further assistance.

Thank you for your reply and the requested information.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Thank you for contacting Abcam.

I am sorry that you experienced difficulties with several of our antibodies in your experiments. I have reviewed the product datasheets and both ab108346 and ab115467 are validated and guaranteed to work in IH...

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