重组
RabMAb

Anti-BCL2L12抗体[EPR5201] (ab108346)

概述

  • 产品名称
    Anti-BCL2L12抗体[EPR5201]
    参阅全部 BCL2L12 一抗
  • 描述
    兔单克隆抗体[EPR5201] to BCL2L12
  • 经测试应用
    适用于: WB, IP, IHC-P, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human BCL2L12.

  • 阳性对照
    • U87-MG, HT-1376, MCF7, and PC-3 cell lysates; Human breast carcinoma tissue IF/ICC: HeLa cell line.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab108346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

靶标

  • 组织特异性
    Expressed mainly in breast, thymus, prostate, fetal liver, colon, placenta, pancreas, small intestine, spinal cord, kidney, and bone marrow and to a lesser extent in many other tissues. Isoform 2 is primarily expressed in skeletal muscle.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • B2L12_HUMAN antibody
    • BCL 2 like 12 (proline rich) antibody
    • BCL 2 like 12 antibody
    • BCL 2 like 12 isoform 1 antibody
    • Bcl 2 like 12 protein antibody
    • Bcl 2 related proline rich protein antibody
    • Bcl-2-like protein 12 antibody
    • Bcl-2-related proline-rich protein antibody
    • BCL2 like 12 (proline rich) antibody
    • BCL2 like 12 antibody
    • BCL2 like 12 isoform 1 antibody
    • Bcl2 like 12 protein antibody
    • Bcl2 related proline rich protein antibody
    • Bcl2-L-12 antibody
    • BCL2L12 antibody
    • BPR antibody
    • MGC120313 antibody
    • MGC120314 antibody
    • MGC120315 antibody
    see all

图片

  • ICC/IF image of ab108346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108346, 1/100) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM

  • Overlay histogram showing HeLa cells stained with ab108346 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108346, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • All lanes : Anti-BCL2L12 antibody [EPR5201] (ab108346) at 1/1000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : HT-1376 cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : PC-3 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 37 kDa
    Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of BCL2L12 in paraffin embedded Human breast carcinoma tissue, using ab108346 at a dilution of 1/500.

文献

This product has been referenced in:
  • Lu W  et al. Long Noncoding RNA LOC100129973 Suppresses Apoptosis by Targeting miR-4707-5p and miR-4767 in Vascular Endothelial Cells. Sci Rep 6:21620 (2016). WB . Read more (PubMed: 26887505) »

See 1 Publication for this product

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Jul 10 2014

You're welcome!

I wish you the best of luck with your research in the future. Please do not hesitate to contact me if I can be of further assistance.

Thank you for your reply and the requested information.

I am sorry these products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To c...

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Thank you for contacting Abcam.

I am sorry that you experienced difficulties with several of our antibodies in your experiments. I have reviewed the product datasheets and both ab108346 and ab115467 are validated and guaranteed to work in IH...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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