重组Anti-Bcl10抗体[EPR3174] (ab108412)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3174] to Bcl10
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Bcl10抗体[EPR3174]
参阅全部 Bcl10 一抗 -
描述
兔单克隆抗体[EPR3174] to Bcl10 -
宿主
Rabbit -
经测试应用
适用于: WB, Flow Cyt (Intra)more details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Daudi and Raji cell lysates; Raji cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR3174 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab108412于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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Flow Cyt (Intra) |
1/500 - 1/1000.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
Flow Cyt (Intra)
1/500 - 1/1000. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
序列相似性
Contains 1 CARD domain. -
翻译后修饰
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
细胞定位
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 8915 Human
- Omim: 603517 Human
- SwissProt: O95999 Human
- Unigene: 193516 Human
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别名
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
图片
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All lanes : Anti-Bcl10 antibody [EPR3174] (ab108412) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
Intracellular flow cytometric analysis of Bcl10 in permeabilized Raji cells, using ab108412 at a 1/500 dilution (red) or a Rabbit IgG (negative) (green).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab108412 尚未被引用在任何文献中。