Anti-Bcl10抗体[4F8E8H8] (ab36585)
Key features and details
- Mouse monoclonal [4F8E8H8] to Bcl10
- Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Bcl10抗体[4F8E8H8]
参阅全部 Bcl10 一抗 -
描述
小鼠单克隆抗体[4F8E8H8] to Bcl10 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, Flow Cyt, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Ni-NTA purified recombinant human BCL-10 expressed in E. Coli strain BL21 (DE3).
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阳性对照
- MCF-7 cell extract. Human breast and liver carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4F8E8H8 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36585于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa).
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Flow Cyt |
1/200.
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ICC/IF |
1/200 - 1/1000.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 34 kDa (predicted molecular weight: 31 kDa). |
Flow Cyt
1/200. |
ICC/IF
1/200 - 1/1000. |
靶标
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功能
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1. -
组织特异性
Ubiquitous. -
疾病相关
Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
Note=Defects in BCL10 are involved in various types of cancer. -
序列相似性
Contains 1 CARD domain. -
翻译后修饰
Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2. -
细胞定位
Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 8915 Human
- Entrez Gene: 12042 Mouse
- Omim: 603517 Human
- SwissProt: O95999 Human
- SwissProt: Q9Z0H7 Mouse
- Unigene: 193516 Human
- Unigene: 239141 Mouse
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别名
- AI132454 antibody
- B cell CLL/lymphoma 10 antibody
- B cell lymphoma/leukemia10 antibody
see all
图片
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All lanes : Anti-Bcl10 antibody [4F8E8H8] (ab36585) at 1/500 dilution
Lane 1 : Cell lysates prepared from NIH/3T3 cells
Lane 2 : Cell lysates prepared from Hela cells
Lane 3 : Cell lysates prepared from MCF-7 cells
Lane 4 : Cell lysates prepared from Jurkat cells
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP-conjugated Goat polyclonal to mouse IgG1
Predicted band size: 31 kDa -
Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human liver carcinoma tissue by immunohistochemistry with DAB staining.
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Ab36585, at a 1/100 - 1/500 dilution, staining Bcl-10 in paraffin embedded human breast carcinoma tissue by immunohistochemistry with DAB staining.
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ab36585 at 1/1000 dilution staining Bcl10 in human Hela cells by Immunocytochemistry/ Immunofluorescence. Bcl10 show green staining in image and actin filament stained red with Alexa Fluor®555 conjugated secondary. Nuclei stained blue with DRAQ5 fluorescent DNA dye.
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Flow cytometric analysis of Hela cells using ab36585 at a 1/200 dilution (green) and negative control (purple).
实验方案
数据表及文件
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Datasheet download
文献 (0)
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