The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
功能Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
疾病相关Note=A chromosomal aberration involving BCL10 is recurrent in low-grade mucosa-associated lymphoid tissue (MALT lymphoma). Translocation t(1;14)(p22;q32). Although the BCL10/IgH translocation leaves the coding region of BCL10 intact, frequent BCL10 mutations could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. Note=Defects in BCL10 are involved in various types of cancer.
序列相似性Contains 1 CARD domain.
翻译后修饰Phosphorylated. Phosphorylation results in dissociation from TRAF2 and binding to BIRC2/c-IAP2.
细胞定位Cytoplasm > perinuclear region. Membrane raft. Appears to have a perinuclear, compact and filamentous pattern of expression. Also found in the nucleus of several types of tumor cells. Colocalized with DPP4 in membrane rafts.
ab125690 at 1/50 dilution staining Bcl10 in Jurkat T cells (2% formaldehyde fixed and permeabilized with 0.1% saponin) by Flow Cytometry (Right image). Left image is stained with a PE-labeled isotope control.