Anti-Bcl-XL抗体[7B2.5] (FITC) (ab26148)

概述

  • 产品名称
    Anti-Bcl-XL抗体[7B2.5] (FITC)
    参阅全部 Bcl-XL 一抗
  • 描述
    小鼠单克隆抗体[7B2.5] to Bcl-XL (FITC)
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm
  • 经测试应用
    适用于: Flow Cyt, IHC-Fr, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Bcl-XL.
    Database link: Q07817

  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

应用

Our Abpromise guarantee covers the use of ab26148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells. ab91539-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 29 kDa.

靶标

  • 功能
    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 组织特异性
    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 序列相似性
    Belongs to the Bcl-2 family.
  • 结构域
    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻译后修饰
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 细胞定位
    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody [7B2.5] (FITC) 图像

  • Flow Cytometry analysis of T cell leukemia cell line Jurkat intracellularly stained with Mouse Anti-Human Bcl-xL-FITC (ab26148) 1 μg/106 cells (purple). Grey - Isotype Control, Mouse IgG3-FITC.

  • Overlay histogram showing DU145 cells stained with ab26148 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab26148, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 (1µg/1x106 cells ). Acquisition of >5,000 events was performed.

Anti-Bcl-XL antibody [7B2.5] (FITC) (ab26148)参考文献

This product has been referenced in:
  • Chong HZ  et al. In vitro evaluation of Pandanus amaryllifolius ethanol extract for induction of cell death on non-hormone dependent human breast adenocarcinoma MDA-MB-231 cell via apoptosis. BMC Complement Altern Med 12:134 (2012). Flow Cyt . Read more (PubMed: 22909149) »
  • Chong HZ  et al. In vitro cytotoxicity of Strobilanthes crispus ethanol extract on hormone dependent human breast adenocarcinoma MCF-7 cell. BMC Complement Altern Med 12:35 (2012). Flow Cyt . Read more (PubMed: 22471785) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I am looking into your question about the intensity of the antibody relative to unstained cells, and will get back to you once I have further information. As to your other question, interestingly enough I was discussin...

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Thank you for your enquiry. I have not heard of a difference between, for example, PE or FITC conjugated antibodies for intracellular staining in FACS. I consulted with several colleagues as well as searched the web and other antibody company's web...

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