Anti-Bcl-XL抗体[2H12] (Biotin) (ab79460)


  • 产品名称Anti-Bcl-XL抗体[2H12] (Biotin)
    参阅全部 Bcl-XL 一抗
  • 描述
    小鼠单克隆抗体[2H12] to Bcl-XL (Biotin)
  • 偶联物Biotin
  • 特异性ab79460 reacts with both isoforms of BCL-XL.
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Pig
    预测可用于: Sheep, Rabbit, Cow, Dog, Chinese Hamster
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-XL aa 3-14.


    Database link: Q07817

  • 阳性对照
    • Hodgkin’s lymphoma tissue.



Our Abpromise guarantee covers the use of ab79460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 2 - 4 µg/ml.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. WB: Use at an assay dependent dilution.

Flow Cyt Use at an assay dependent concentration.

Use at an assay dependent dilution.

ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.


  • 功能Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.
  • 组织特异性Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.
  • 翻译后修饰Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.
  • 细胞定位Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

Anti-Bcl-XL antibody [2H12] (Biotin) (ab79460)参考文献

This product has been referenced in:
  • Hsu YT & Youle RJ Nonionic detergents induce dimerization among members of the Bcl-2 family. J Biol Chem 272:13829-34 (1997). Read more (PubMed: 9153240) »
  • Hsu YT  et al. Cytosol-to-membrane redistribution of Bax and Bcl-X(L) during apoptosis. Proc Natl Acad Sci U S A 94:3668-72 (1997). Read more (PubMed: 9108035) »

See all 3 Publications for this product

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Thank you for your enquiry and your interest in our products. Neither of these two antibodies has been tested in rabbit, though due to sequence homology the cross-reactivity with this species is expected. It is important to mention that both ab54396...

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