The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明IHC-P: 1/50 - 1/100 in an ABC method. An incubation period of 30 minutes at room temperature is recommended. Formalin fixed, paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6 prior to immunostaining.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
相关性Bcl x is a member of the Bcl 2 family of proteins, which function as regulators of apoptosis. It may provide neuroprotection against ischemic brian injury. Bcl x has two isoforms, Bcl xl (long) a 241 amino acid protein and Bcl xs (short) a 178 amino acid protein lacking a 63 amino acid domain that is well conserved among members of the Bcl2 family. Isoform Bcl-X(L) anti-apoptotic activity is inhibited by association with SIVA isoform 1. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, cytochrome c, from the mitochondrial membrane. The Bcl-X(S) isoform promotes apoptosis.