Bcl G overexpression 293T裂解液(全细胞) (ab94328)

概述

  • 产品名称Bcl G overexpression 293T裂解液(全细胞)
  • 描述
    Bcl G overexpression裂解液
  • 常规说明ab94328 is a 293T cell transfected lysate in which Human Bcl G has been transiently over-expressed using a pCMV-Bcl G plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • 经测试应用适用于: WBmore details

性能

  • 背景Bcl-G is a novel pro-apoptotic member of the Bcl-2 family. The human Bcl-G gene encodes two proteins through alternative mRNA splicing, Bcl-G(L) (long) and Bcl-G(S) (short) consisting of 327 and 252 amino acids in length, respectively. Bcl-G(L) mRNA is expressed widely in adult human tissues, whereas Bcl-G(S) mRNA was found only in testis. Overexpression of Bcl-G(L) or Bcl-G(S) in cells induced apoptosis although Bcl-G(S) was far more potent than Bcl-G(L).

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应用

Our Abpromise guarantee covers the use of ab94328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

Bcl G overexpression 293T lysate (whole cell) 图像

  • ab94328 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-Bcl G antibody (ab57742) at 1/500 dilution

    Lane 1 : Bcl G overexpression 293T lysate (whole cell) (ab94328)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

Bcl G overexpression 293T lysate (whole cell) (ab94328)参考文献

ab94328 has not yet been referenced specifically in any publications.

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