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Our Abpromise guarantee covers the use of ab28819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19738029
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Western blot analysis of lysates from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the phospho peptide.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"