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Our Abpromise guarantee covers the use of ab28819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/100.|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 19738029ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Western blot analysis of lysates from HeLa cells treated with LPS (40nM, 30mins), K562 cells treated with calyculin A (50ng/ml, 30mins) and COS-7 cells treated with H2O2 (1ng/ml, 15mins), using BCL-2 (Phospho-Ser70) Antibody. The lane on the right is blocked with the phospho peptide.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"