Anti-Bcl-2抗体[Bcl2/100] (ab117115)

概述

  • 产品名称Anti-Bcl-2抗体[Bcl2/100]
    参阅全部 Bcl-2 一抗
  • 描述
    小鼠单克隆抗体[Bcl2/100] to Bcl-2
  • 经测试应用适用于: WB, IP, IHC-P, IHC-Fr, ICC, Flow Cytmore details
  • 种属反应性
    与反应: Dog, Human
    预测可用于: Cow, Cat, Pig不与反应: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-2 aa 41-54.
    Sequence:

    GAAPAPGIFSSQPG


    Database link: P10415

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab117115 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • 组织特异性Expressed in a variety of tissues.
  • 疾病相关A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • 翻译后修饰Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • 细胞定位Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator Bcl 2 antibody
    • Apoptosis regulator Bcl-2 antibody
    • Apoptosis regulator Bcl2 antibody
    • AW986256 antibody
    • B cell CLL/lymphoma 2 antibody
    • B cell leukemia/lymphoma 2 antibody
    • Bcl-2 antibody
    • Bcl2 antibody
    • BCL2_HUMAN antibody
    • C430015F12Rik antibody
    • D630044D05Rik antibody
    • D830018M01Rik antibody
    • Leukemia/lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    • PPP1R50 antibody
    • Protein phosphatase 1, regulatory subunit 50 antibody
    see all

Anti-Bcl-2 antibody [Bcl2/100] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of dog breast cancer tissue sections labeling Bcl-2 with ab117115 at 1/50 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using EDTA. An undiluted polyclonal mouse biotinylated secondary antibody was used.

    See Abreview

Anti-Bcl-2 antibody [Bcl2/100] (ab117115)参考文献

This product has been referenced in:
  • Wu J  et al. Silencing of Kv1.5 Gene Inhibits Proliferation and Induces Apoptosis of Osteosarcoma Cells. Int J Mol Sci 16:26914-26 (2015). WB ; Human . Read more (PubMed: 26569226) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Breast cancer)
Specification Breast cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA
Permeabilization No
Blocking step Novocastra Peroxidase Block as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
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Verified customer

提交于 Jul 13 2012

Thank you for your reply.

Thank you for agreeing to test these products in IHC-P in dog samples. We look forward to receiving your Abreviews.

Please don't hesitate to contact me should you have further questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"