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Our Abpromise guarantee covers the use of ab117115 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 26 kDa.|
|IP||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of dog breast cancer tissue sections labeling Bcl-2 with ab117115 at 1/50 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using EDTA. An undiluted polyclonal mouse biotinylated secondary antibody was used.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"