• 产品名称Anti-Bcl-2抗体
    参阅全部 Bcl-2 一抗
  • 描述
    兔多克隆抗体to Bcl-2
  • 特异性This antibody reacts with Bcl-2
  • 经测试应用适用于: WB, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human Bcl-2 (internal sequence).
    Database link: P10415

  • 阳性对照
    • A20 cell lysate and A20 cells



Our Abpromise guarantee covers the use of ab62468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
ICC Use a concentration of 2 µg/ml.


  • 功能Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785).
  • 组织特异性Expressed in a variety of tissues.
  • 疾病相关A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
    The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
    The loop between motifs BH4 and BH3 is required for the interaction with NLRP1.
  • 翻译后修饰Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
    Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.
  • 细胞定位Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator Bcl 2 antibody
    • Apoptosis regulator Bcl-2 antibody
    • Apoptosis regulator Bcl2 antibody
    • AW986256 antibody
    • B cell CLL/lymphoma 2 antibody
    • B cell leukemia/lymphoma 2 antibody
    • Bcl-2 antibody
    • Bcl2 antibody
    • BCL2_HUMAN antibody
    • C430015F12Rik antibody
    • D630044D05Rik antibody
    • D830018M01Rik antibody
    • Leukemia/lymphoma, B-cell, 2 antibody
    • Oncogene B-cell leukemia 2 antibody
    • PPP1R50 antibody
    • Protein phosphatase 1, regulatory subunit 50 antibody
    see all

Anti-Bcl-2 antibody 图像

  • Lane 1 : Anti-Bcl-2 antibody (ab62468) at 1 µg/ml
    Lane 2 : Anti-Bcl-2 antibody (ab62468) at 2 µg/ml
    Lane 3 : Anti-Bcl-2 antibody (ab62468) at 4 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate
    Lane 3 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    Anti rabbit IgG secondary antibody

    Predicted band size : 26 kDa
    Observed band size : 26 kDa
  • Immunocytochemistry of Bcl-2 in A20 cells with ab62468 antibody at 2 µg/ml.

Anti-Bcl-2 antibody (ab62468)参考文献

ab62468 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry. I am sorry to confirm that we do not sell any antibodies that have been directed to this specific region of the Bcl2 protein. However, we do have the following sheep polyclonal ab16120 Bcl2 antibody. This has been ra...

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