Anti-Bcl-2抗体(ab194583)
Key features and details
- Rabbit polyclonal to Bcl-2
- Suitable for: IHC-P, WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Bcl-2抗体
参阅全部 Bcl-2 一抗 -
描述
兔多克隆抗体to Bcl-2 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IF, IPmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Bcl-2 aa 1-100.
Database link: P10415 -
阳性对照
- WB: HL-60 cell lysate, mouse testis, rat lung and rat liver tissue lysates; IHC: Human lung cancer, rat brain and mouse testis; ICC/ IF: C6, NIH-3T3 and U-2 OS cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab194583于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/50 - 1/100.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/500 - 1/2000. Predicted molecular weight: 26 kDa.
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ICC/IF |
1/50 - 1/100.
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IP |
1/1000.
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说明 |
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IHC-P
1/50 - 1/100. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Predicted molecular weight: 26 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/1000. |
靶标
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功能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
组织特异性
Expressed in a variety of tissues. -
疾病相关
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
细胞定位
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 596 Human
- Entrez Gene: 12043 Mouse
- Entrez Gene: 24224 Rat
- Omim: 151430 Human
- SwissProt: P10415 Human
- SwissProt: P10417 Mouse
- SwissProt: P49950 Rat
- Unigene: 150749 Human
see all -
别名
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
图片
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All lanes : Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1 : 239T cell lysate
Lane 2 : 239T Bcl-2 knock-out cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s. -
All lanes : Anti-Bcl-2 antibody (ab194583) at 1/500 dilution
Lane 1 : Mouse lung cell extract
Lane 2 : Mouse spleen cell extract
Lane 3 : Rat spleen cell extract
Lane 4 : Rat ovary cell extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 26 kDaBlocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic KitExposure time: 30 seconds
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Immunoprecipitation analysis of 200ug extracts of THP-1 cells using 3ug Bcl-2 antibody (ab194583). Western blot was performed from the immunoprecipitate using Bcl-2 antibody (ab194583) at a dilition of 1:1000.
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Immunofluorescence analysis of U-2 OS cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of C6 cells using Bcl-2 Rabbit pAb (A0208) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunohistochemistry of paraffin-embedded human appendix using Bcl-2 Rabbit pAb (ab194583) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (107)
ab194583 被引用在 107 文献中.
- Liu L et al. Hypercholesterolemia aggravates sevoflurane-induced cognitive impairment in aged rats by inducing neurological inflammation and apoptosis. J Biochem Mol Toxicol 36:e23009 (2022). PubMed: 35174938
- Zhuang J et al. Rosuvastatin exerts cardioprotective effect in lipopolysaccharide-mediated injury of cardiomyocytes in an MG53-dependent manner. BMC Cardiovasc Disord 22:69 (2022). PubMed: 35196979
- Shi X et al. Circ_0037866 Contributes to the Tumorigenesis of Renal Cell Carcinoma by Sequestering miR-384 to Elevate Chromobox 5 Expression. Kidney Blood Press Res 47:329-340 (2022). PubMed: 35249038
- Yang Q & Long F MiRNA-384-5p targets GABRB1 to regulate ketamine-Induced Neurotoxicity in Neurons. Turk Neurosurg N/A:N/A (2022). PubMed: 35416273
- Wang S et al. Pathological Changes and Expression of JAK-STAT Signaling Pathway Hallmark Proteins in Rat Retinas at Different Time Points After Retinal Ischemia Reperfusion Injury. Pathol Oncol Res 28:1610385 (2022). PubMed: 35515015