Anti-BCKDHA抗体(ab68094)

概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to BCKDHA
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human, Caenorhabditis elegans
  • 免疫原

    Full length protein, corresponding to amino acids 1-445 of Human BCKDHA

  • 阳性对照
    • Transfected 293T cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG

应用

Our Abpromise guarantee covers the use of ab68094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能
      The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
    • 疾病相关
      Maple syrup urine disease 1A
    • 序列相似性
      Belongs to the BCKDHA family.
    • 细胞定位
      Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
      • FLJ45695 antibody
      • OVD1A antibody
      • 2 oxoisovalerate dehydrogenase (lipoamide) antibody
      • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • BCKDE1A antibody
      • BCKDH E1 alpha antibody
      • BCKDH E1-alpha antibody
      • BCKDHA antibody
      • Branched chain alpha keto acid dehydrogenase E1 component alpha chain antibody
      • Branched chain keto acid dehydrogenase E1 alpha polypeptide antibody
      • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) antibody
      • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody
      • MSU antibody
      • MSUD1 antibody
      • ODBA_HUMAN antibody
      see all

    图片

    • All lanes : Anti-BCKDHA antibody (ab68094) at 1/500 dilution

      Lane 1 : BCKDHA transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 50 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    实验方案

    文献

    ab68094 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Sample
    Caenorhabditis elegans Tissue lysate - whole (Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu)
    Specification
    Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Julianne Martell

    Verified customer

    提交于 Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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