Anti-BCKDHA抗体(ab68094)

概述

  • 产品名称Anti-BCKDHA抗体
    参阅全部 BCKDHA 一抗
  • 描述
    小鼠多克隆抗体to BCKDHA
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human, Caenorhabditis elegans
  • 免疫原

    Full length protein, corresponding to amino acids 1-445 of Human BCKDHA

  • 阳性对照
    • Transfected 293T cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG

应用

Our Abpromise guarantee covers the use of ab68094 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 50 kDa).
    Detection pair

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).
    • 疾病相关Maple syrup urine disease 1A
    • 序列相似性Belongs to the BCKDHA family.
    • 细胞定位Mitochondrion matrix.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
      • FLJ45695 antibody
      • OVD1A antibody
      • 2 oxoisovalerate dehydrogenase (lipoamide) antibody
      • 2 oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial antibody
      • BCKDE1A antibody
      • BCKDH E1 alpha antibody
      • BCKDH E1-alpha antibody
      • BCKDHA antibody
      • Branched chain alpha keto acid dehydrogenase E1 component alpha chain antibody
      • Branched chain keto acid dehydrogenase E1 alpha polypeptide antibody
      • Branched chain keto acid dehydrogenase E1, alpha polypeptide (maple syrup urine disease) antibody
      • Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain antibody
      • MSU antibody
      • MSUD1 antibody
      • ODBA_HUMAN antibody
      see all

    Anti-BCKDHA antibody 图像

    • All lanes : Anti-BCKDHA antibody (ab68094) at 1/500 dilution

      Lane 1 : BCKDHA transfected 293T cell lysate
      Lane 2 : non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 50 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-BCKDHA antibody (ab68094)参考文献

    ab68094 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing (10% gel)
    Sample Caenorhabditis elegans Tissue lysate - whole (Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu)
    Specification Used on DAF-2 (CB1370) and DAF-2/DAF-16(DR1309) mu
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Julianne Martell

    Verified customer

    提交于 Nov 11 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"