Anti-BCKDH kinase抗体(ab65027)

概述

  • 产品名称Anti-BCKDH kinase抗体
    参阅全部 BCKDH kinase 一抗
  • 描述
    兔多克隆抗体to BCKDH kinase
  • 特异性ab65027 detects endogenous levels of total BCKDH kinase protein.
  • 经测试应用适用于: ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from an internal sequence of human BCKDH kinase

  • 阳性对照
    • 293 and Jurkat cell extracts and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab65027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

靶标

  • 功能Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Anti-BCKDH kinase antibody 图像

  • ab65027 at 1/500 - 1/1000 dilution staining BCKDH kinase in HeLa cells by Immunofluorescence, in the absence (-) or presence (+) of the immunising peptide.
  • All lanes : Anti-BCKDH kinase antibody (ab65027) at 1/500 dilution

    Lane 1 : 293 cell extract
    Lane 2 : Jurkat cell extract
    Lane 3 : Jurkat cell extract with immunising peptide


    Predicted band size : 46 kDa
    Observed band size : 46 kDa

Anti-BCKDH kinase antibody (ab65027)参考文献

ab65027 has not yet been referenced specifically in any publications.

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