Anti-BCKDH kinase抗体(ab111716)

概述

  • 产品名称Anti-BCKDH kinase抗体
    参阅全部 BCKDH kinase 一抗
  • 描述
    兔多克隆抗体to BCKDH kinase
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 46-287 of Human BCKDH kinase (AAH07363).

  • 阳性对照
    • 293T and Mouse brain whole cell lysates; Human gastric carcinoma tissue.

性能

应用

Our Abpromise guarantee covers the use of ab111716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)

靶标

  • 功能Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
  • 组织特异性Ubiquitous.
  • 序列相似性Belongs to the PDK/BCKDK protein kinase family.
    Contains 1 histidine kinase domain.
  • 翻译后修饰Autophosphorylated.
  • 细胞定位Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • [3 methyl 2 oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial antibody
    • [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase antibody
    • BCKD kinase antibody
    • BCKD-kinase antibody
    • BCKD_HUMAN antibody
    • BCKDHKIN antibody
    • Bckdk antibody
    • BCKDKD antibody
    • BDK antibody
    • Branched chain alpha keto acid dehydrogenase kinase antibody
    • Branched chain ketoacid dehydrogenase kinase antibody
    • Branched-chain alpha-ketoacid dehydrogenase kinase antibody
    • mitochondrial antibody
    see all

Anti-BCKDH kinase antibody 图像

  • Anti-BCKDH kinase antibody (ab111716) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 46 kDa
  • Anti-BCKDH kinase antibody (ab111716) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 46 kDa
  • ab111716 at 1/500 dilution staining BCKDH kinase in Formalin-fixed, paraffin-embedded Human Gastric carcinoma tissue by Immunohistochemistry.

Anti-BCKDH kinase antibody (ab111716)参考文献

ab111716 has not yet been referenced specifically in any publications.

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