The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
May promote cell cycle arrest by enhancing the inhibition of CDK2 activity by CDKN1A. May be required for repair of DNA damage by homologous recombination in conjunction with BRCA2. May not be involved in non-homologous end joining (NHEJ).
Expressed at high levels in testis and skeletal muscle and at lower levels in brain, heart, kidney, liver, lung, ovary, pancreas, placenta, and spleen.
Belongs to the BCP1 family.
Isoform 1 is expressed throughout the cell cycle. Isoform 2 is expressed following mitosis and peaks in the G1/S phase of the cell cycle.
Nucleus. Colocalizes with BRCA2 in discrete nuclear foci.
ab113288, at 1/100 dilution, staining BCCIP in formalin-fixed, paraffin-embedded Human breast carcinoma by Immunohistochemistry. Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100.