Anti-BCAT1抗体(ab125209)
Key features and details
- Rabbit polyclonal to BCAT1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BCAT1抗体
参阅全部 BCAT1 一抗 -
描述
兔多克隆抗体to BCAT1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide from the C terminal region of Human BCAT1
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阳性对照
- IHC-P: Human breast tissue. WB: HeLa and Jurkat cell lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125209于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa.
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IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa. |
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. -
组织特异性
During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma. -
序列相似性
Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 586 Human
- Omim: 113520 Human
- SwissProt: P54687 Human
- Unigene: 438993 Human
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别名
- BCAT antibody
- BCAT(c) antibody
- Bcat1 antibody
see all
图片
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All lanes : Anti-BCAT1 antibody (ab125209) at 0.5 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 43 kDa -
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BCAT1 with ab125209 at 0.5 μg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab125209 被引用在 1 文献中.
- Belone Ade F et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870