The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
Use at 2-5 µg/mg of lysate.
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
Belongs to the WD repeat BCAS3 family. Contains 3 WD repeats.
Immunoprecipitation - Breast carcinoma amplified sequence 3 antibody (ab71162)
Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
Lane 1: ab71162 at 3µg/mg whole cell lysate.
Lane 2: Control IgG.
All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
Detection: Chemiluminescence with an exposure time of 1 second.