Anti-BCAS3抗体(ab71162)

概述

  • 产品名称Anti-BCAS3抗体
    参阅全部 BCAS3 一抗
  • 描述
    兔多克隆抗体to BCAS3
  • 经测试应用适用于: WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rhesus monkey, Orangutan
  • 免疫原

    A synthetic peptide corresponding to a region between residue 870 and the C terminus (residue 928) of human Breast carcinoma amplified sequence 3 (BAB15156.1)

  • 阳性对照
    • Whole cell lysate from MCF7 cells.

性能

应用

Our Abpromise guarantee covers the use of ab71162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 101 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 组织特异性Expressed in stomach, liver, lung, kidney, prostate, testis, thyroid gland, adrenal gland, brain, heart, skeletal muscle, colon, spleen, small intestine, placenta, blood leukocyte and mammary epithelial cells. Overexpressed in most breast cancer cell lines.
  • 疾病相关Note=A chromosomal aberration involving BCAS3 has been found in some breast carcinoma cell lines. Translocation t(17;20)(q23;q13) with BCAS4.
  • 序列相似性Belongs to the WD repeat BCAS3 family.
    Contains 3 WD repeats.
  • 发展阶段Fetal.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1500019F07Rik antibody
    • 2610028P08Rik antibody
    • AU021018 antibody
    • BC028339 antibody
    • BCAS3 antibody
    • BCAS3_HUMAN antibody
    • Breast carcinoma-amplified sequence 3 antibody
    • FLJ20128 antibody
    • GAOB1 antibody
    • K20D4 antibody
    • MAAB antibody
    • Maab1 protein antibody
    • MGC4973 antibody
    • rudhira antibody
    see all

Anti-BCAS3 antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling BCAS3 with ab71162 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-BCAS3 antibody (ab71162) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from MCF7 cells at 50 µg
    Lane 2 : Whole cell lysate from MCF7 cells at 15 µg
    Lane 3 : Whole cell lysate from MCF7 cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 101 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • Immunoprecipitation/ Western Blot of Breast carcinoma amplified sequence 3.
    Lane 1: ab71162 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    All lanes: Whole cell lysate from MCF7 cells at 1mg for IP, 20% of IP loaded.
    Detection: Chemiluminescence with an exposure time of 1 second.

Anti-BCAS3 antibody (ab71162)参考文献

ab71162 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Melanoma Cell Line)
Total protein in input 1000 µg
Immuno-precipitation step Protein A
Specification Melanoma Cell Line
Username

Mr. John Finnigan

Verified customer

提交于 Apr 26 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"