Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Catfish, Orangutan
Synthetic peptide corresponding to a region between residue 175 and the C-terminus (residue 225) of human BCAS2 (NP_005863.1)
Whole cell lysate from MCF7 cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 26 kDa.
Use at 2-5 µg/mg of lysate.
1/2000 - 1/10000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in mRNA splicing.
Belongs to the SPF27 family.
Nucleus > nucleolus.
Information by UniProt
Breast carcinoma amplified sequence 2 antibody
Breast carcinoma-amplified sequence 2 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAS2 antibody (ab71161)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small cell lung cancer (left) and mouse squamous cell carcinoma (right) tissues labelling BCAS2 at 1/5000 (0.2µg/ml). Detection: DAB.
Immunoprecipitation - Anti-BCAS2 antibody (ab71161)
Detection of Human BCAS2 by Immunoprecipitation in Whole cell lysate from MCF7 cells (1 mg for IP, 20% of IP loaded) using ab71161 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
has not yet been referenced specifically in any publications.
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