Anti-BCAR3/NSP2抗体(ab85940)
Key features and details
- Rabbit polyclonal to BCAR3/NSP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-BCAR3/NSP2抗体 -
描述
兔多克隆抗体to BCAR3/NSP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human BCAR3/NSP2 aa 1-100. NP_003558.1
Database link: O75815 -
阳性对照
- HeLa and 293T whole cell lysates
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
ab85940 was affinity purified using an epitope specific to BCAR3/NSP2 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85940于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
说明 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
-
功能
May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins. -
组织特异性
Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue. -
序列相似性
Contains 1 Ras-GEF domain.
Contains 1 SH2 domain. - Information by UniProt
-
数据库链接
- Entrez Gene: 8412 Human
- Omim: 604704 Human
- SwissProt: O75815 Human
- Unigene: 36958 Human
-
别名
- BCAR 3 antibody
- BCAR3 antibody
- BCAR3_HUMAN antibody
see all
图片
-
All lanes : Anti-BCAR3/NSP2 antibody (ab85940) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 300 kDa, 460 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Detection of human BCAR3/NSP2 in Immunoprecipitates of whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85940 at 3 µg/mg lysate for IP, and at 1 µg/ml for subsequent Western blot.
Detection: Chemiluminescence with an exposure time of 10 seconds.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab85940 尚未被引用在任何文献中。