概述

  • 产品名称Anti-BCAR3抗体
    参阅全部 BCAR3 一抗
  • 描述
    兔多克隆抗体to BCAR3
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from the C-terminal of human BCAR3.

  • 阳性对照
    • Human colon carcinoma tissue. Extracts from HeLa, Jurkat and HUVEC cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab65111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).
ELISA 1/10000.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
  • 组织特异性Ubiquitously expressed. Found in several cancer cell lines, but not in nonmalignant breast tissue.
  • 序列相似性Contains 1 Ras-GEF domain.
    Contains 1 SH2 domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCAR 3 antibody
    • BCAR3 antibody
    • BCAR3_HUMAN antibody
    • Breast cancer anti estrogen resistance 3 antibody
    • Breast cancer anti estrogen resistance protein 3 antibody
    • Breast cancer anti-estrogen resistance protein 3 antibody
    • Breast cancer antiestrogen resistance 3 antibody
    • dJ1033H22.2 antibody
    • dJ1033H22.2 breast cancer anti estrogen resistance 3 antibody
    • dJ1033H22.2 breast cancer antiestrogen resistance 3 antibody
    • KIAA0554 antibody
    • Novel SH2 containing protein 2 antibody
    • Novel SH2-containing protein 2 antibody
    • NSP 2 antibody
    • NSP2 antibody
    • SH2 containing protein Nsp2 antibody
    • SH2 domain containing protein 3B antibody
    • SH2 domain-containing protein 3B antibody
    • SH2D3B antibody
    see all

Anti-BCAR3 antibody 图像

  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue stained with BCAR3 antibody (ab65111) at 1/50 - 1/100 dilution, with (right) and without (left) the immunising peptide.
  • All lanes : Anti-BCAR3 antibody (ab65111) at 1/500 dilution

    Lane 1 : extracts from HeLa cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HUVEC cells
    Lane 4 : extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 93 kDa
    Observed band size : 93 kDa
  • ICC/IF image of ab65111 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65111, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BCAR3 antibody (ab65111)参考文献

ab65111 has not yet been referenced specifically in any publications.

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