Anti-BCAR1 (phospho Y762)抗体(ab41827)
Key features and details
- Rabbit polyclonal to BCAR1 (phospho Y762)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BCAR1 (phospho Y762)抗体
参阅全部 BCAR1 一抗 -
描述
兔多克隆抗体to BCAR1 (phospho Y762) -
宿主
Rabbit -
特异性
This antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated BCAR1 on SDS-PAGE immunoblots of pervanadate treated human A431 cells, but not in control cells. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Rat BCAR1 (phospho Y762).
Database link: Q63767 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-BCAR1(Tyr-751) peptide before affinity purification using phospho-BCAR1(Tyr-762) peptide (without carrier). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab41827于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/2000. Predicted molecular weight: 130 kDa.
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说明 |
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WB
1/2000. Predicted molecular weight: 130 kDa. |
靶标
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功能
Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells. -
组织特异性
Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line. -
序列相似性
Belongs to the CAS family.
Contains 1 SH3 domain. -
结构域
Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
A serine-rich region promotes activation of the serum response element (SRE).
The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11. -
翻译后修饰
PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
Dephosphorylated by PTPN14 at Tyr-128. -
细胞定位
Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation. - Information by UniProt
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数据库链接
- Entrez Gene: 9564 Human
- Omim: 602941 Human
- SwissProt: P56945 Human
- Unigene: 479747 Human
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别名
- BCAR 1 antibody
- Bcar1 antibody
- BCAR1_HUMAN antibody
see all
图片
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Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4-6). The blot was probed with anti-BCAR1 (lanes 1 & 2) or anti-BCAR1 (Tyr-762) (lanes 3-6). The latter was used in the presence of no peptide (lane 4), phospho-p130 Cas (Tyr-762) peptide (lane 5) or phospho-BCAR1 (Tyr-751) peptide (lane 6).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab41827 被引用在 1 文献中.
- Zheng Y et al. Protein-tyrosine Kinase 6 Promotes Peripheral Adhesion Complex Formation and Cell Migration by Phosphorylating p130 CRK-associated Substrate. J Biol Chem 287:148-58 (2012). ICC/IF ; Human . PubMed: 22084245