Anti-BCAR1 (phospho Y751)抗体(ab45486)

概述

  • 产品名称Anti-BCAR1 (phospho Y751)抗体
    参阅全部 BCAR1 一抗
  • 描述
    兔多克隆抗体to BCAR1 (phospho Y751)
  • 特异性The antibody detects a 130 kDa protein corresponding to the molecular mass of phosphorylated p130 Cas on SDS-PAGE immunoblots of pervanadate treated human endothelial and A431 cells, but is not observed in control cells.
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse, Human
  • 免疫原

    Phospho-p130 Cas (Tyr-751) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 751 in rat p130 Cas. .

  • 阳性对照
    • Pervanadate treated human endothelial and A431 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to phospho-p130 Cas (Tyr-762) peptide before affinity purification using phospho-p130 Cas (Tyr-751) peptide (without carrier).
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab45486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 93 kDa.
ELISA 1/2000.

靶标

  • 功能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • 组织特异性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • 序列相似性Belongs to the CAS family.
    Contains 1 SH3 domain.
  • 结构域Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • 翻译后修饰PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • 细胞定位Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Anti-BCAR1 (phospho Y751) antibody 图像

  • Lanes 1 - 2 : anti-p130 Cas
    Lanes 3 - 4 : Anti-BCAR1 (phospho Y751) antibody (ab45486) at 1/2000 dilution
    Lanes 5 - 6 : anti-p130 Cas (Tyr-762)

    Lane 1 : human endothelial cells serum starved overnight
    Lane 2 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
    Lane 3 : human endothelial cells serum starved overnight
    Lane 4 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes
    Lane 5 : human endothelial cells serum starved overnight
    Lane 6 : human endothelial cells treated with pervanadate (1 mM) for 30 minutes


    Predicted band size : 93 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)

Anti-BCAR1 (phospho Y751) antibody (ab45486)参考文献

ab45486 has not yet been referenced specifically in any publications.

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