Anti-BCAR1 (phospho Y410)抗体(ab55263)

概述

  • 产品名称Anti-BCAR1 (phospho Y410)抗体
    参阅全部 BCAR1 一抗
  • 描述
    兔多克隆抗体to BCAR1 (phospho Y410)
  • 特异性ab55263 detects endogenous levels of BCAR1 only when phosphorylated at tyrosine 410.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic phosphopeptide derived from BCAR1 around the phosphorylation site of tyrosine 410 (G-V-YP-A-V).

  • 阳性对照
    • human breast carcinoma tissue, extracts from NIH/3T3 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab55263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 94 kDa (predicted molecular weight: 94 kDa).
ELISA 1/5000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • 组织特异性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • 序列相似性Belongs to the CAS family.
    Contains 1 SH3 domain.
  • 结构域Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • 翻译后修饰PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • 细胞定位Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Anti-BCAR1 (phospho Y410) antibody 图像

  • All lanes : Anti-BCAR1 (phospho Y410) antibody (ab55263) at 1/500 dilution

    Lane 1 : extracts from NIH/3T3 cells.
    Lane 2 : extracts from NIH/3T3 cells, incubated with the immunising phosphopeptide.


    Predicted band size : 94 kDa
    Observed band size : 94 kDa
  • Immunohistochemical analysis of BCAR1 (phospho Y410) expression in paraffin embedded human breast carcinoma tissue, using 1/50 ab55263. Left: untreated sample. Right: sample treated with immunising phosphopeptide.

Anti-BCAR1 (phospho Y410) antibody (ab55263)参考文献

ab55263 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab55263.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"