概述

  • 产品名称Anti-BCAR1抗体
    参阅全部 BCAR1 一抗
  • 描述
    兔多克隆抗体to BCAR1
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human BCAR1.

    (Peptide available as ab89387.)

  • 阳性对照
    • This antibody gave a positive signal in the following tissue lysates: Human Brain; Human Testis; Human Liver. Whole cell lysates: MBA MD 231; MDA MB 361; MCF7; T47D. This antibody gave a positive result in IHC in the following FFPE tissue: Human Testis.

性能

应用

Our Abpromise guarantee covers the use of ab80016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
  • 组织特异性Widely expressed with an abundant expression in the testis. Low level of expression seen in the liver, thymus, and peripheral blood leukocytes. The protein has been detected in a B-cell line.
  • 序列相似性Belongs to the CAS family.
    Contains 1 SH3 domain.
  • 结构域Contains a central domain (substrate domain) containing multiple potential SH2-binding sites and a C-terminal domain containing a divergent helix-loop-helix (HLH) motif. The SH2-binding sites putatively bind CRK, NCK and ABL1 SH2 domains. The HLH motif is absolutely required for the induction of pseudohyphal growth in yeast and mediates heterodimerization with NEDD9.
    A serine-rich region promotes activation of the serum response element (SRE).
    The SH3 domain is necessary for the localization of the protein to focal adhesions and interacts with one proline-rich region of PTK2/FAK11.
  • 翻译后修饰PTK2/FAK1 activation mediates phosphorylation at the YDYVHL motif; phosphorylation is most likely catalyzed by SRC family members. SRC-family kinases are recruited to the phosphorylated sites and can phosphorylate other tyrosine residues. Tyrosine phosphorylation is triggered by integrin-mediated adhesion of cells to the extracellular matrix.
    Dephosphorylated by PTPN14 at Tyr-128.
  • 细胞定位Cell junction, focal adhesion. Cytoplasm. Unphosphorylated form localizes in the cytoplasm and can move to the membrane upon tyrosine phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BCAR 1 antibody
    • Bcar1 antibody
    • BCAR1_HUMAN antibody
    • Breast cancer anti estrogen resistance 1 antibody
    • Breast cancer anti estrogen resistance 1 protein antibody
    • Breast cancer anti-estrogen resistance protein 1 antibody
    • CAS antibody
    • Cas scaffolding protein family member 1 antibody
    • CAS1 antibody
    • Cass1 antibody
    • Crk associated substrate antibody
    • Crk associated substrate p130Cas antibody
    • CRK-associated substrate antibody
    • CRKAS antibody
    • FLJ12176 antibody
    • FLJ45059 antibody
    • p130cas antibody
    see all

Anti-BCAR1 antibody 图像

  • IHC image of BCAR1 staining in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab80016, 0.1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-BCAR1 antibody (ab80016) at 1 µg/ml

    Lane 1 : Human breast tissue lysate - total protein (ab30090)
    Lane 2 : Human testis tissue lysate - total protein (ab30257)
    Lane 3 : Human liver tissue lysate - total protein (ab29889)
    Lane 4 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 5 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : T47D whole cell lysate (ab14899)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 93 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
    Breast cancer anti-estrogen resistance protein 1 (BCAR1) contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher (100 kDa) molecular weight than predicted (93 kDa).
  • ICC/IF image of ab80016 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80016, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HEK293, HepG2, and MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HEK293, HepG2, and MCF-7 cells at 1µg/ml.

Anti-BCAR1 antibody (ab80016)参考文献

ab80016 has not yet been referenced specifically in any publications.

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