概述

  • 产品名称
  • 描述
    兔多克隆抗体to BCA1
  • 经测试应用
    适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    A synthetic peptide corresponding to a sequence at the C-terminal of Human BCA1.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Monobasic dihydrogen sodium phosphate
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab112521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 13 kDa. Recombinant tested only. The detection limit for BCA1 is approximately 2.5ng/lane under non-reducing and reducing conditions.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Chemotactic for B-lymphocytes but not for T-lymphocytes, monocytes and neutrophils. Does not induce calcium release in B-lymphocytes. Binds to BLR1/CXCR5.
  • 组织特异性
    Highest levels in liver, followed by spleen, lymph node, appendix and stomach. Low levels in salivary gland, mammary gland and fetal spleen.
  • 序列相似性
    Belongs to the intercrine alpha (chemokine CxC) family.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ANGIE antibody
    • ANGIE2 antibody
    • B cell attracting chemokine 1 antibody
    • B cell-attracting chemokine 1 antibody
    • B lymphocyte chemoattractant antibody
    • B-cell chemoattractant antibody
    • B-cell-attracting chemokine 1 antibody
    • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1) antibody
    • BCA-1 antibody
    • BLC antibody
    • BLR1L antibody
    • C-X-C motif chemokine 13 antibody
    • Chemokine (C-X-C motif) ligand 13 antibody
    • Chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant) antibody
    • Chemokine, CXC motif, ligand 13 antibody
    • CXC chemokine BLC antibody
    • CXCL13 antibody
    • CXL13_HUMAN antibody
    • SCYB13 antibody
    • Small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cell chemoattractant) antibody
    • Small inducible cytokine B13 antibody
    • Small inducible cytokine subfamily B, member 13 antibody
    • Small-inducible cytokine B13 antibody
    see all

图片

  • ab112521 staining BCA1 in Rat brain tissue sections by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 1% in PBS and blocked with 2% BSA for 20 minutes at 21°C. Samples were incubated with primary antibody (1/1000 in PBS-T) for 12 hours. An Alexa Fluor®594-conjugated Donkey anti-rabbit polyclonal(1/1000) was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-BCA1 antibody (ab112521)

    Lane 1 : Recombinant human BCA1 protein at 0.01 µg
    Lane 2 : Recombinant human BCA1 protein at 0.005 µg
    Lane 3 : Recombinant human BCA1 protein at 0.0025 µg


    Predicted band size : 13 kDa
    Observed band size : 11 kDa (why is the actual band size different from the predicted?)

文献

This product has been referenced in:
  • Tomas-Sanchez C  et al. Prophylactic Chronic Zinc Administration Increases Neuroinflammation in a Hypoxia-Ischemia Model. J Immunol Res 2016:4039837 (2016). ELISA ; Rat . Read more (PubMed: 27635404) »
  • Lee HS  et al. Comparative gene-expression analysis of the dental follicle and periodontal ligament in humans. PLoS One 8:e84201 (2013). IHC-P ; Human . Read more (PubMed: 24376796) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunocytochemistry
Sample
Human Cultured Cells (dental cell)
Permeabilization
Yes - 0.25% Triton X-100
Specification
dental cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 30 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
None
Sample
Human Tissue sections (Follicle)
Specification
Follicle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Apr 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (tooth)
Specification
tooth
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Mar 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Specification
Spleen
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 May 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (brain tissue)
Specification
brain tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - tritonx100
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Apr 10 2013

For ab112521: DISCOUNT CODE:***

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I am very pleased to hear you would like to accept our offer and test bothab112521 and ab133706 in IHC-Fr. This code will give you: 1 free antibody per code before the expiration date. To redeem this offer, please submit an Abreview for IHC-F...

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Thank you very much for your interest in our products.

I have contacted the lab regarding additional information about this two antibodies.

ab133706 Anti-CXCR5 antibody [EPR8837] was tested in mouse and rat tissue lysates: brain, hea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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