概述

  • 产品名称Anti-BBX抗体
    参阅全部 BBX 一抗
  • 描述
    兔多克隆抗体to BBX
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 864-911 (SAFFSLAALA EVAAMENVHR GQRSTPLTHD GQPKEMPQAP VLISCADQ) of Human BBX (NP_064620).

  • 阳性对照
    • COLO205 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab94418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-BBX antibody 图像

  • Anti-BBX antibody (ab94418) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 102 kDa

Anti-BBX antibody (ab94418)参考文献

ab94418 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Tissue lysate - whole (whole embryos)
Specification whole embryos
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Evgenia Salta

Verified customer

提交于 Feb 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"