概述

  • 产品名称Anti-BBS4抗体
    参阅全部 BBS4 一抗
  • 描述
    小鼠多克隆抗体to BBS4
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length BBS4 protein (Human)

  • 阳性对照
    • BBS4 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab67189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 58 kDa).
    Detection Antibody Pair.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required for microtubule anchoring at the centrosome but not for microtubule nucleation. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8(GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane.
    • 组织特异性Ubiquitously expressed. The highest level of expression is found in the kidney.
    • 疾病相关Defects in BBS4 are the cause of Bardet-Biedl syndrome type 4 (BBS4) [MIM:209900]. Bardet-Biedl syndrome (BBS) is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation. Secondary features include diabetes mellitus, hypertension and congenital heart disease. A relatively high incidence of BBS is found in the mixed Arab populations of Kuwait and in Bedouin tribes throughout the Middle East, most likely due to the high rate of consaguinity in these populations and a founder effect.
    • 序列相似性Belongs to the BBS4 family.
      Contains 10 TPR repeats.
    • 细胞定位Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton. Cell projection > cilium membrane. Cytoplasm. Localizes to the pericentriolar region throughout the cell cycle. Centrosomal localization requires dynein. Localizes to nonmembranous centriolar satellites in the cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Bardet Biedl syndrome 4 protein antibody
      • Bardet-Biedl syndrome 4 protein antibody
      • Bbs4 antibody
      • BBS4_HUMAN antibody

    Anti-BBS4 antibody 图像

    • All lanes : Anti-BBS4 antibody (ab67189) at 1/500 dilution

      Lane 1 : BBS4 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg/ml per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size : 58 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    Anti-BBS4 antibody (ab67189)参考文献

    ab67189 has not yet been referenced specifically in any publications.

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