概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to BBS2
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Recombinant fragment
    预测可用于: Human
  • 免疫原

    Full length BBS2 protein (Human)

  • 阳性对照
    • BBS2 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab68223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).

靶标

  • 相关性
    Defects in BBS2 are the cause of Bardet-Biedl syndrome type 2. Bardet-Biedl syndrome is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation.
  • 细胞定位
    Cytoplasmic
  • 数据库链接
  • 别名
    • Bardet Biedl syndrome 2 antibody
    • Bardet Biedl syndrome 2 protein antibody
    • BBS antibody
    • MGC20703 antibody

图片

  • All lanes : Anti-BBS2 antibody (ab68223) at 1/500 dilution

    Lane 1 : BBS2 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 80 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab68223 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Monkey Cell lysate - whole cell (kidney cells)
Loading amount
20 µg
Specification
kidney cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

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提交于 Aug 31 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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