Anti-Bax inhibitor 1抗体(ab18852)

概述

  • 产品名称Anti-Bax inhibitor 1抗体
    参阅全部 Bax inhibitor 1 一抗
  • 描述
    兔多克隆抗体to Bax inhibitor 1
  • 特异性ab18852 recognises Bax inhibitor.
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (Human) of Bax inhibitor 1.

  • 阳性对照
    • rat testis tissue lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: 50% Glycerol, 1% BSA, PBS, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab18852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 26 kDa.

靶标

Anti-Bax inhibitor 1 antibody 图像

  • Anti-Bax inhibitor 1 antibody (ab18852) at 1 µg/ml + rat testis tissue lysate

    Predicted band size : 26 kDa
    Observed band size : 22 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab18852 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18852, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Bax inhibitor 1 antibody (ab18852)参考文献

ab18852 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"