概述

  • 产品名称Anti-Bax抗体[E63]
    参阅全部 Bax 一抗
  • 描述
    兔单克隆抗体[E63] to Bax
  • 经测试应用适用于: IHC-P, WB, IP, Sandwich ELISAmore details
    不适用于: Flow Cyt or ICC
  • 种属反应性
    与反应: Mouse, Rat, Human, Chinese Hamster
    预测可用于: Cow
  • 免疫原

    Synthetic peptide within Human Bax aa 1-100 (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: HeLa cell lysate. IHC-P: Human lymph node and rat kidney tissues.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab32503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IP 1/100.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [2D2] to Bax (ab77566).
  • 应用说明Is unsuitable for Flow Cyt or ICC.
  • 靶标

    • 功能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
    • 组织特异性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
    • 序列相似性Belongs to the Bcl-2 family.
    • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
    • 细胞定位Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    Anti-Bax antibody [E63] 图像



    • Predicted band size : 21 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: Bax knockout HAP1 cell lysate (20 µg)

      Lanes 1 - 2: Merged signal (red and green). Green - ab32503 observed at 20 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32503 was shown to recognize Bax when Bax knockout samples were used, along with additional cross-reactive bands. Wild-type and Bax knockout samples were subjected to SDS-PAGE. ab32503 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • IHC image of ab32503 staining Bax in rat kidney formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32503, 1:250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the secondary only control (shown on the inset).
      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



    • Predicted band size : 21 kDa

      Lane 1 = Bax protein (Tagged) (ab85157), 10 ng. Lane 2 = Extract of HeLa cells, 40 ug. Lane 3 = Extract of HepG2 cells, 40 ug. Lane 4 = Bax protein (Tagged) (ab85157), 10 ng. Lane 5 = Extract of HeLa cells, 40 ug. Lane 6 = Extract of HepG2 cells, 40 ug. SDS PAGE performed under reducing conditions (100mM DTT Sample heated at 50oC). Primary : Lanes 1-3: Anti Bax antibody (ab77566) at 1 ug/mL. Lanes 4-6: Anti Bax antibody (ab32503) at 1:2000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 2 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : Bax 21kDa and Bax (Tagged) 49 kDa. Observed band size : Bax 21kDa and Bax (Tagged) 49 kDa.

    • Anti-Bax antibody [E63] (ab32503) at 1/2000 dilution + HeLa cell lysate

      Secondary
      Goat anti-rabbit HRP antibody
      Developed using the ECL technique

      Predicted band size : 21 kDa
    • Immunohistochemical analysis of paraffin-embedded Human lymph node using anti-Bax Rabbit Monoclonal Antibody (ab32503) at 1/250 dilution.

    • Standard Curve for Bax (Analyte: ab85157) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [2D2] to Bax - BSA and Azide free (ab77566) at 0.2ug/ml and Detector Antibody Rabbit monoclonal [E63] to Bax (ab32503) at 0.5ug/ml Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.

    实验方案

    Anti-Bax antibody [E63] (ab32503)参考文献

    This product has been referenced in:
    • He G  et al. Gadd45b prevents autophagy and apoptosis against rat cerebral neuron oxygen-glucose deprivation/reperfusion injury. Apoptosis 21:390-403 (2016). WB ; Rat . Read more (PubMed: 26882903) »
    • Chen B  et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB ; Mouse . Read more (PubMed: 26848028) »

    See all 47 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (Injured spinal cord)
    Antigen retrieval step None
    Permeabilization Yes - Triton-X100
    Specification Injured spinal cord
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative Paraformaldehyde
    Username

    清隆 新井

    Verified customer

    提交于 Sep 19 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Dog Tissue sections (Tonsil)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer ph6.0
    Permeabilization No
    Specification Tonsil
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 26°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Sep 01 2016

    Application Western blot
    Sample Chinese Hamster Cell lysate - whole cell (CHO cell lines)
    Gel Running Conditions Reduced Denaturing (4-20%)
    Loading amount 5 µg
    Specification CHO cell lines
    Blocking step Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Username

    Abcam user community

    Verified customer

    提交于 Mar 11 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (primary)
    Specification primary
    Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative Methanol
    Username

    Abcam user community

    Verified customer

    提交于 Apr 20 2015

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Cell lysate - whole cell (MEF)
    Specification MEF
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Rat Cell lysate - whole cell (h9c2)
    Specification h9c2
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application Western blot
    Loading amount 30 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (HEK293)
    Specification HEK293
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Mouse Cell (MEF)
    Specification MEF
    Permeabilization Yes - 1% TRITON-X-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Rat Cell (h9c2)
    Specification h9c2
    Permeabilization Yes - 1% TRITON-X-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 14 2014

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Sample Human Cell (HEK293)
    Specification HEK293
    Permeabilization Yes - 1% TRITON-X-100
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Jul 08 2014

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