概述

  • 产品名称Anti-Bax抗体
    参阅全部 Bax 一抗
  • 描述
    兔多克隆抗体to Bax
  • 经测试应用适用于: ELISA, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide corresponding to Human Bax (internal sequence).
    Database link: Q07812

  • 阳性对照
    • Human liver carcinoma tissue, 293 cell extract and HUVEC cells.

性能

应用

Our Abpromise guarantee covers the use of ab69643 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/20000.
WB 1/500 - 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/100 - 1/500.

靶标

  • 功能Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 组织特异性Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • 序列相似性Belongs to the Bcl-2 family.
  • 结构域Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 细胞定位Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Apoptosis regulator BAX antibody
    • BAX antibody
    • Bax-protein antibody
    • BAX_HUMAN antibody
    • BAXA antibody
    • Baxdelta2G9 antibody
    • Baxdelta2G9omega antibody
    • Baxdelta2omega antibody
    • Bcl-2-like protein 4 antibody
    • BCL2 associated X protein antibody
    • BCL2 associated X protein omega antibody
    • BCL2 associated X protein transcript variant delta2 antibody
    • Bcl2-L-4 antibody
    • BCL2L4 antibody
    • membrane isoform alpha antibody
    see all

Anti-Bax antibody 图像

  • Immunofluorescence analysis of HUVEC cells, staining Bax with ab69643 at 1/100 dilution.

  • All lanes : Anti-Bax antibody (ab69643) at 1/500 dilution

    Lane 1 : 293 cell extracts
    Lane 2 : 293 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa
    Observed band size : 21 kDa
    Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded Human liver carcinoma tissue staining BAX with ab69643 at 1/50 dilution. The image on the right has been treated with the immunizing peptide.

Anti-Bax antibody (ab69643)参考文献

This product has been referenced in:
  • Dinh C  et al. Short Interfering RNA against Bax Attenuates TNFa-Induced Ototoxicity in Rat Organ of Corti Explants. Otolaryngol Head Neck Surg 148:834-40 (2013). ICC/IF ; Rat . Read more (PubMed: 23401256) »
  • Monsalve DM  et al. Human VRK2 modulates apoptosis by interaction with Bcl-xL and regulation of BAX gene expression. Cell Death Dis 4:e513 (2013). Human . Read more (PubMed: 23449449) »

See all 3 Publications for this product

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