概述

  • 产品名称Anti-BAT3抗体
    参阅全部 BAT3 一抗
  • 描述
    小鼠多克隆抗体to BAT3
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length Human BAT3 (NP_542433.1).

  • 阳性对照
    • A-431 cell lysate with BAT3 expression, BAT3 transfected 293T cell lysate and HeLa cells.

性能

应用

Our Abpromise guarantee covers the use of ab88292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 119 kDa.
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Chaperone that plays a key role in various processes such as apoptosis, insertion of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane and regulation of chromatin. Acts in part by regulating stability of proteins and their degradation by the proteasome. Participates in endoplasmic reticulum stress-induced apoptosis via its interaction with AIFM1/AIF by regulating AIFM1/AIF stability and preventing its degradation. Also required during spermatogenesis for synaptonemal complex assembly via its interaction with HSPA2, by inhibiting polyubiquitination and subsequent proteasomal degradation of HSPA2. Required for selective ubiquitin-mediated degradation of defective nascent chain polypeptides by the proteasome. In this context, may play a role in immuno-proteasomes to generate antigenic peptides via targeted degradation, thereby playing a role in antigen presentation in immune response. Key component of the BAG6/BAT3 complex, a cytosolic multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. BAG6/BAT3 acts by facilitating TA membrane proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins and transfers them to ASNA1/TRC40 for targeting to the endoplasmic reticulum membrane. Also involved in DNA damage-induced apoptosis: following DNA damage, accumulates in the nucleus and forms a complex with p300/EP300, enhancing p300/EP300-mediated p53/TP53 acetylation leading to increase p53/TP53 transcriptional activity. When nuclear, may also act as a component of some chromatin regulator complex that regulates histone 3 'Lys-4' dimethylation (H3K4me2).
  • 序列相似性Contains 1 ubiquitin-like domain.
  • 翻译后修饰Cleavage by caspase-3 releases a C-terminal peptide that plays a role in ricin-induced apoptosis.
    In case of infection by L.pneumophila, ubiquitinated by the SCF(LegU1) complex.
  • 细胞定位Cytoplasm > cytosol. Nucleus. The C-terminal fragment generated by caspase-3 is cytoplasmic.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2410045D21Rik antibody
    • AA408914 antibody
    • BAG 6 antibody
    • BAG family molecular chaperone regulator 6 antibody
    • BAG-6 antibody
    • BAG6 antibody
    • BAG6_HUMAN antibody
    • BAT 3 antibody
    • BCL2-associated athanogene 6 antibody
    • D17H6S52E antibody
    • D6S52E antibody
    • G3 antibody
    • HLA B associated transcript 3 antibody
    • HLA-B associated transcript 3 antibody
    • HLA-B associated transcript-3 antibody
    • HLA-B-associated transcript 3 antibody
    • large proline rich protein BAG6 antibody
    • Large proline rich protein BAT3 antibody
    • Large proline-rich protein BAG6 antibody
    • large proline-rich protein BAT3 antibody
    • Protein G3 antibody
    • Protein Scythe antibody
    • Scythe antibody
    • Scythe, homolog of Xenopus antibody
    see all

Anti-BAT3 antibody 图像

  • Anti-BAT3 antibody (ab88292) at 1 µg/ml + A-431 cell lysate with BAT3 expression at 50 µg

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 119 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-BAT3 antibody (ab88292) at 1 µg/ml

    Lane 1 : BAT3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG at 1/2500 dilution

    Predicted band size : 119 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence of ab88292 on HeLa cell with antibody concentration at 10 ug/ml
  • IHC image of ab88292 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab88292, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-BAT3 antibody (ab88292)参考文献

This product has been referenced in:

See all 2 Publications for this product

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