Anti-BarX1抗体(ab26156)

概述

  • 产品名称Anti-BarX1抗体
    参阅全部 BarX1 一抗
  • 描述
    兔多克隆抗体to BarX1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chicken, Cow, Dog不与反应: Mouse
  • 免疫原

    Synthetic peptide derived from a region between residues 59 - 108 of human BarX1.

    .

  • 阳性对照
    • HepG2 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab26156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

靶标

  • 相关性BarX1 belongs to the Bar subclass of the homeobox gene family. The function of this gene has not yet been determined; however, studies in the mouse and chick homolog suggest a role in developing teeth and craniofacial mesenchyme of neural crest origin. The role of these homologs implicates the human gene as a candidate for unmapped disorders involving tooth and jaw development.
  • 细胞定位Nuclear
  • 数据库链接
  • 别名
    • BarH like homeobox 1 antibody
    • BarX 1 antibody
    • Homeobox protein BarH like 1 antibody

Anti-BarX1 antibody 图像

  • Anti-BarX1 antibody (ab26156) at 0.5 µg/ml + HepG2 cell lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50000 - 1/100000

    Predicted band size : 24 kDa
  • Paraffin embeded Tissue: Human Heart
    Cells with positive label: Myocardial cells (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X
  • Paraffin embeded Tissue: Human Pancreas
    Cells with positive label: Epithelial cells of pancreatic acinus (indicated with arrows)
    Antibody concentration:4-8µg/ml
    Magnification:400X

Anti-BarX1 antibody (ab26156)参考文献

ab26156 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (tooth)
Antigen retrieval step None
Permeabilization No
Specification tooth
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 19 2015

Thank you for your reply. It is unfortunate that the sequence information for pig is not available. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on...

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Thank you for your enquiry. To our knowledge, the antibody has yet to be tested in pig. All tested species are listed on Abcam product datasheets. I am, however, enquiring with the originator of this product to find out the homology between the imm...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (mandible, digestive tract, stomach)
Loading amount 30 µg
Specification mandible, digestive tract, stomach
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Isabelle Miletich

Verified customer

提交于 Oct 25 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"