The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
The Drosophila BarH1 and BarH2 (Bar) genes are homeobox containing genes, which are required for the fate determination of external sensory organs in the fly. Using a bioinformatic approach, murine and human homeobox genes highly related to the Bar Drosophila genes were identified, which were designated Barhl1 and Barhl2. Murine Barhl2 is the homolog of the rat mBH1 gene. The human BARHL2 fragment shares 92% and 74% nucleotide identity, respectively, with the rat mBH1 and human BARHL1 cDNA sequences.