概述

  • 产品名称Anti-BARD1抗体
    参阅全部 BARD1 一抗
  • 描述
    兔多克隆抗体to BARD1
  • 特异性ab69645 detects endogenous levels of total BARD1 protein.
  • 经测试应用适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from N-terminal of human BARD1.

  • 阳性对照
    • HeLa cell extracts.

性能

应用

Our Abpromise guarantee covers the use of ab69645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 80 kDa (predicted molecular weight: 86 kDa).

靶标

  • 功能Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • 细胞定位Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all

Anti-BARD1 antibody 图像

  • All lanes : Anti-BARD1 antibody (ab69645) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 86 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

Anti-BARD1 antibody (ab69645)参考文献

ab69645 has not yet been referenced specifically in any publications.

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