Anti-BARD1抗体(ab115477)

概述

  • 产品名称Anti-BARD1抗体
    参阅全部 BARD1 一抗
  • 描述
    兔多克隆抗体to BARD1
  • 经测试应用适用于: ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog
  • 免疫原

    Synthetic peptide:

    CSKLRNLLHDNELSDLK

    conjugated to KLH, corresponding to amino acids 108-124 of Human BARD1.

  • 阳性对照
    • Human skeletal muscle and skin tissue

性能

应用

Our Abpromise guarantee covers the use of ab115477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1 - 1/1000.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
  • 通路Protein modification; protein ubiquitination.
  • 序列相似性Contains 4 ANK repeats.
    Contains 2 BRCT domains.
    Contains 1 RING-type zinc finger.
  • 翻译后修饰Processed during apoptosis. The homodimer is more susceptible to proteolytic cleavage than the BARD1/BRCA1 heterodimer.
  • 细胞定位Nucleus. During S phase of the cell cycle, colocalizes with BRCA1 into discrete subnuclear foci. Can translocate to the cytoplasm. Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BARD-1 antibody
    • Bard1 antibody
    • BARD1_HUMAN antibody
    • BRCA1 associated RING domain 1 antibody
    • BRCA1 associated RING domain gene 1 antibody
    • BRCA1 associated RING domain protein 1 antibody
    • BRCA1-associated RING domain protein 1 antibody
    see all

Anti-BARD1 antibody 图像

  • ab115477, at 10µg/ml, staining BARD1 in formalin-fixed, paraffin-embedded Human skeletal muscle tissue by Immunohistochemistry, using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115477, at 10µg/ml, staining BARD1 in formalin-fixed, paraffin-embedded Human skin tissue by Immunohistochemistry, using a biotinylated anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-BARD1 antibody (ab115477)参考文献

ab115477 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (EC109)
Gel Running Conditions Non-reduced Denaturing (12% SDS)
Loading amount 80 µg
Treatment CHX for indicated time points
Specification EC109
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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提交于 Dec 31 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"