The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IP: Use at a concentration of 1 - 10 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
BAPTA ((1,2-bis(2-Aminophenoxy)ethane-N,N,N',N'- tetraacetic acid) is a highly selective calcium chelator. BAPTA has spawned many of the most familiar fluorescent indicators for calcium and other ions. BAPTA and its analogs including BAPTA-AM have proven most critical in cell physiology research where they have been essential in defining the mechanisms
underlying inter- or intracellular calcium homeostasis and calcium dependent exocytosis. BAPTA and its analogs may also find application in the study of diseases in which the regulation of excitatory
neurotransmitter release and uptake has been disturbed.