重组Anti-BAP31抗体[EPR3878(2)] (ab109304)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3878(2)] to BAP31
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BAP31抗体[EPR3878(2)]
参阅全部 BAP31 一抗 -
描述
兔单克隆抗体[EPR3878(2)] to BAP31 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- WB: HEK293T, NCCIT and HeLa cell lysates; Human placenta tissue lysate. IHC-P: Human kidney tissue and Human brain tissue. ICC/IF: HeLa cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR3878(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab109304于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 28 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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说明 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 28 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
靶标
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功能
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. -
组织特异性
Ubiquitous. -
疾病相关
Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1. -
序列相似性
Belongs to the BCAP29/BCAP31 family. -
翻译后修饰
Cleaved by CASP8 and other caspases. -
细胞定位
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex. - Information by UniProt
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数据库链接
- Entrez Gene: 10134 Human
- Entrez Gene: 27061 Mouse
- Omim: 300398 Human
- SwissProt: P51572 Human
- SwissProt: Q61335 Mouse
- Unigene: 522817 Human
- Unigene: 17 Mouse
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别名
- 6C6 AG antibody
- 6C6 AG tumor associated antigen antibody
- 6C6-AG tumor-associated antigen antibody
see all
图片
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All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : BCAP31 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : NCCIT cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDaLanes 1-4: Merged signal (red and green). Green - ab109304 observed at 28 kDa. Red - loading control ab7291 observed at 50 kDa.
ab109304 Anti-BAP31 antibody [EPR3878(2)] was shown to specifically react with BAP31 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266634 (knockout cell lysate ab257857) was used. Wild-type and BAP31 knockout samples were subjected to SDS-PAGE. ab109304 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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ab109304, at 1/250 dilution, staining Human kidney tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab109304, at 1/100 dilution, staining HeLa cells by immunofluorescence.
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All lanes : Anti-BAP31 antibody [EPR3878(2)] (ab109304) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human placenta tissue lysate
Lane 3 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 28 kDa -
ab109304, at 1/250 dilution, staining Human brain tissue by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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