Anti-BAP31抗体[7A3BB6] (ab112993)


  • 产品名称Anti-BAP31抗体[7A3BB6]
    参阅全部 BAP31 一抗
  • 描述
    小鼠单克隆抗体[7A3BB6] to BAP31
  • 特异性Shotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • 经测试应用适用于: IHC-P, ICC/IF, WB, IP, In-Cell ELISA, Flow Cytmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Hela cell fraction

  • 阳性对照
    • HepG2 cell lysate; HL60 and Human fibroblast cells. Human normal colon FFPE tissue.
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • 存储溶液Preservative: 0.02% Sodium azide
    Constituent: 99.98% HBS
  • Concentration information loading...
  • 纯度>95% by SDS-PAGE
  • 纯化说明ab112993 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE.
  • 克隆单克隆
  • 克隆编号7A3BB6
  • 同种型IgG1
  • 轻链类型kappa
  • 研究领域


Our Abpromise guarantee covers the use of ab112993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 µg/ml. Predicted molecular weight: 28 kDa.
IP Use at an assay dependent concentration.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • 功能May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis.
  • 组织特异性Ubiquitous.
  • 疾病相关Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1.
  • 序列相似性Belongs to the BCAP29/BCAP31 family.
  • 翻译后修饰Cleaved by CASP8 and other caspases.
  • 细胞定位Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6C6 AG antibody
    • 6C6 AG tumor associated antigen antibody
    • 6C6-AG tumor-associated antigen antibody
    • 6C6AG antibody
    • 6C6AG tumor associated antigen antibody
    • Accessory protein BAP 31 antibody
    • Accessory protein BAP31 antibody
    • B cell receptor associated protein 31 antibody
    • B-cell receptor-associated protein 31 antibody
    • BA31 antibody
    • BAP 31 antibody
    • Bap31 antibody
    • BAP31_HUMAN antibody
    • BCAP 31 antibody
    • BCAP31 antibody
    • BCR associated protein Bap 31 antibody
    • BCR associated protein Bap31 antibody
    • BCR-associated protein 31 antibody
    • CDM antibody
    • CDM protein antibody
    • DXS1357E antibody
    • MS950 antibody
    • p28 antibody
    • p28 Bap31 antibody
    • Protein CDM antibody
    • RP23-329M9.5 antibody
    see all

Anti-BAP31 antibody [7A3BB6] 图像

  • All lanes : Anti-BAP31 antibody [7A3BB6] (ab112993) at 0.5 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : H9C2 Cell Lysate
    Lane 3 : MEF Cell Lysate

    Predicted band size : 28 kDa
  • ab112993 at 1ug/ml staining BAP31 in MeOH-fixed HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • ab112993 at 5 µg/ml staining BAP31 in Human fibroblast cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly in the endoplasmic reticulum.
  • IHC image of BAP31 staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112993, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-BAP31 antibody [7A3BB6] (ab112993)参考文献

ab112993 has not yet been referenced specifically in any publications.

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