Anti-BAP1抗体(ab167250)
Key features and details
- Mouse polyclonal to BAP1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BAP1抗体
参阅全部 BAP1 一抗 -
描述
小鼠多克隆抗体to BAP1 -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to amino acids 1-729 of Human BAP1 (NP_004647.1).
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阳性对照
- BAP1 transfected 293T cell lysate; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab167250于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Deubiquitinating enzyme that plays a key role in chromatin by mediating deubiquitination of histone H2A and HCFC1. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-119' (H2AK119ub1). Does not deubiquitinate monoubiquitinated histone H2B. Acts as a regulator of cell growth by mediating deubiquitination of HCFC1 N-terminal and C-terminal chains, with some specificity toward 'Lys-48'-linked polyubiquitin chains compared to 'Lys-63'-linked polyubiquitin chains. Deubiquitination of HCFC1 does not lead to increase stability of HCFC1. Interferes with the BRCA1 and BARD1 heterodimer activity by inhibiting their ability to mediate ubiquitination and autoubiquitination. It however does not mediate deubiquitination of BRCA1 and BARD1. Acts as a tumor suppressor. -
组织特异性
Highly expressed in testis, placenta and ovary. Expressed in breast. -
序列相似性
Belongs to the peptidase C12 family. BAP1 subfamily. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Cytoplasm. Nucleus. Mainly nuclear. Binds to chromatin. - Information by UniProt
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数据库链接
- Entrez Gene: 8314 Human
- Omim: 603089 Human
- SwissProt: Q92560 Human
- Unigene: 106674 Human
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别名
- BAP 1 antibody
- Bap1 antibody
- BAP1_HUMAN antibody
see all
图片
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All lanes : Anti-BAP1 antibody (ab167250) at 1 µg
Lane 1 : BAP1 transfected 293T cell lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 15 µl per lane.
Developed using the ECL technique.
Predicted band size: 80 kDa -
Immunofluorescence analysis of HeLa cells labeling BAP1 with ab167250 at 10µg/mL.
实验方案
数据表及文件
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Datasheet download
文献 (1)
ab167250 被引用在 1 文献中.
- Sahtoe DD et al. BAP1/ASXL1 recruitment and activation for H2A deubiquitination. Nat Commun 7:10292 (2016). PubMed: 26739236