antigen sequence: FLSEPIGEKD VCWVDGISHE LAINLVTKGI NKAYILLGQF LLMHKNEAEF QRWLICCFGA TECEAQQTSH CLKEWCA, corresponding to amino acids 11-87 of Human BANF2.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration.
Use a concentration of 1 - 4 µg/ml.
May play a role in BANF1 regulation and influence tissue-specific roles of BANF1.
Expressed strongly in testis and pancreas. Also detected in brain, colon, liver, lung, ovary, placenta, prostate, small intestine, spleen and thymus. Not detected in heart, kidney and skeletal muscle.
Belongs to the BAF family.
Information by UniProt
Western blot - Anti-BANF2 antibody (ab122806)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF2 antibody (ab122806)
ab122806, at 1/50 dilution, staining BANF2 in paraffin-embedded Human pancreas tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence-Anti-BANF2 antibody(ab122806)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli using ab122806.
Recommended treatment: Fixation/Permeabilization: PFA/Triton X-100
has not yet been referenced specifically in any publications.
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