概述

  • 产品名称Anti-BANF1抗体
    参阅全部 BANF1 一抗
  • 描述
    小鼠单克隆抗体to BANF1
  • 经测试应用适用于: WB, ELISA, ICC/IF, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Recombinant full length BANF1 protein (Human) aas 1-90, with a 26 kDa tag, AAH05942.

  • 阳性对照
    • Recombinant protein (immunogen), Jurkat cell lysate and HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab88464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt 1/50. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
  • 组织特异性Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the BAF family.
  • 结构域Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
    LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
  • 翻译后修饰Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAF antibody
    • BAF_HUMAN antibody
    • BANF 1 antibody
    • BANF1 antibody
    • Barrier to autointegration factor 1 antibody
    • Barrier to autointegration factor antibody
    • Barrier-to-autointegration factor antibody
    • BCRG 1 antibody
    • BCRG1 antibody
    • BCRP 1 antibody
    • BCRP1 antibody
    • Breakpoint cluster region protein 1 antibody
    • D14S1460 antibody
    • MGC111161 antibody
    • NGPS antibody
    see all

Anti-BANF1 antibody 图像

  • Anti-BANF1 antibody (ab88464) at 5 µg/ml + recombinant protein (immunogen) at 0.2 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

    Predicted band size : 10 kDa
    Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  • Anti-BANF1 antibody (ab88464) at 5 µg/ml + Jurkat cell lysate at 25 µg

    Secondary
    Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 10 kDa
    Observed band size : 8 kDa (why is the actual band size different from the predicted?)
  • ab88464 at 10µg/ml staining BANF1 in Hela cells.
  • Overlay histogram showing HepG2 cells stained with ab88464 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab88464, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-BANF1 antibody (ab88464)参考文献

This product has been referenced in:
  • Paquet N  et al. Néstor-Guillermo Progeria Syndrome: a biochemical insight into Barrier-to-Autointegration Factor 1, alanine 12 threonine mutation. BMC Mol Biol 15:27 (2014). WB . Read more (PubMed: 25495845) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 myoblast)
Loading amount 10 µg
Specification C2C12 myoblast
Gel Running Conditions Reduced Denaturing (16%)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

提交于 Feb 22 2012

Thank you for your participation in Abcam's follow up survey.

Please let me know how the free of charge replacement vial worked for you - once you have tried it.

If there is anything else I can do for you, or if you have questions t...

Read More

We do not have any information about the new lot, other than that it has passed quality control. We have not received any complaints about it. The new lot is ****. If you do place another order for ab88464, you will want to specify that you want this p...

Read More

Thank you for sending your protocol details. The one suggestion I have that may improve the signal strength is to switch the membrane blocking buffer from 5% milk to 1% BSA. The antigen recognition of different batches of polyclonal antibodies is often...

Read More

Thank you for bringing this to our attention. I am sorry to read that you are having difficulty with these vials. Please send a few details about your samples (tissue type and species and how the samples were prepared), and the protocol. We would like ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"