概述

  • 产品名称Anti-BANF1抗体
    参阅全部 BANF1 一抗
  • 描述
    小鼠单克隆抗体to BANF1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein: MTTSQKHRDF VAEPMGEKPV GSLAGIGEVL GKKLEERGFD KAYVVLGQFL VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL, corresponding to amino acids 1-90 of Human BANF1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆单克隆
  • 同种型IgG2a
  • 轻链类型kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab56517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 10 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
  • 组织特异性Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the BAF family.
  • 结构域Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
    LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides.
  • 翻译后修饰Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
  • 细胞定位Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAF antibody
    • BAF_HUMAN antibody
    • BANF 1 antibody
    • BANF1 antibody
    • Barrier to autointegration factor 1 antibody
    • Barrier to autointegration factor antibody
    • Barrier-to-autointegration factor antibody
    • BCRG 1 antibody
    • BCRG1 antibody
    • BCRP 1 antibody
    • BCRP1 antibody
    • Breakpoint cluster region protein 1 antibody
    • D14S1460 antibody
    • MGC111161 antibody
    • NGPS antibody
    see all

Anti-BANF1 antibody 图像

  • BANF1 antibody (ab56517) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human esophagus.


  • Predicted band size : 10 kDa
    BANF1 antibody (ab56517) at 1ug/lane + Jurkat cell lysate at 25ug/lane.
  • ICC/IF image of ab56517 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56517, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-BANF1 antibody (ab56517)参考文献

This product has been referenced in:
  • Gerner C  et al. Indications for cell stress in response to adenoviral and baculoviral gene transfer observed by proteome profiling of human cancer cells. Electrophoresis 31:1822-32 (2010). WB ; Human . Read more (PubMed: 20446292) »
  • Chan EY  et al. Dynamic host energetics and cytoskeletal proteomes in human immunodeficiency virus type 1-infected human primary CD4 cells: analysis by multiplexed label-free mass spectrometry. J Virol 83:9283-95 (2009). WB ; Human . Read more (PubMed: 19587052) »

See all 2 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (H293)
Loading amount 100000 cells
Specification H293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Jul 17 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"