概述
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产品名称Anti-BANF1抗体
参阅全部 BANF1 一抗 -
描述兔多克隆抗体to BANF1
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经测试应用适用于: WB, IHC-P, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse
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免疫原
Synthetic peptide containing 15 amino acids from near the C terminus of BANF1 (Human) conjugated to KLH
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阳性对照
- Human heart tissue
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab115226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 10 kDa. | |
| IHC-P | Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
靶标
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功能Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD.
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组织特异性Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes.
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序列相似性Belongs to the BAF family.
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结构域Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides. -
翻译后修饰Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA.
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细胞定位Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein.
- Information by UniProt
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数据库链接
- Entrez Gene: 8815 Human
- Entrez Gene: 23825 Mouse
- Omim: 603811 Human
- SwissProt: O75531 Human
- SwissProt: O54962 Mouse
- Unigene: 433759 Human
- Unigene: 358649 Mouse
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别名
- BAF antibody
- BAF_HUMAN antibody
- BANF 1 antibody
see all
Anti-BANF1 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BANF1 antibody (ab115226)Human heart (formalin fixed, paraffin embedded) stained with ab115226 at 5 ug/ml followed by biotinylated Goat anti Rabbit IgG secondary antibody, alkaline phosphatase streptavidin and chromogen.
实验方案
Anti-BANF1 antibody (ab115226)参考文献
ab115226 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"