RabMAb

Anti-Band 3 (phospho Y359)抗体[EPR1427Y] (ab77236)

概述

  • 产品名称Anti-Band 3 (phospho Y359)抗体[EPR1427Y]
    参阅全部 Band 3 一抗
  • 描述
    兔单克隆抗体[EPR1427Y] to Band 3 (phospho Y359)
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding tyrosine 359 of human Band 3.

  • 阳性对照
    • Red blood cell lysates treated with pervanadate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab77236 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/50000. Predicted molecular weight: 102 kDa.
  • 应用说明Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • 组织特异性Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • 疾病相关Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • 序列相似性Belongs to the anion exchanger (TC 2.A.31) family.
    • 翻译后修饰Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • 细胞定位Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Anti-Band 3 (phospho Y359) antibody [EPR1427Y] 图像

    • All lanes : Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236) at 1/50000 dilution

      Lane 1 : Red blood cell lysate, untreated.
      Lane 2 : Red blood cell lysate treated with pervanadate.

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 102 kDa

    Anti-Band 3 (phospho Y359) antibody [EPR1427Y] (ab77236)参考文献

    ab77236 has not yet been referenced specifically in any publications.

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    The macaca Band 3 protein is about 94% homologous to the human protein. It seems that the anti human Band 3 antibodies will cross react with Macaca proteins also.

    You can choose any of the anti Band 3 a...

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    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytopl...

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