RabMAb

Anti-Band 3抗体[EPR1426] (ab108414)

概述

  • 产品名称Anti-Band 3抗体[EPR1426]
    参阅全部 Band 3 一抗
  • 描述
    兔单克隆抗体[EPR1426] to Band 3
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Band 3 aa 1-100.
    (Peptide available as ab177142)

  • 阳性对照
    • Red blood cell lysate; Human colon and lung tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 100 kDa (predicted molecular weight: 102 kDa).
IHC-P 1/100 - 1/250.
ICC/IF Use at an assay dependent concentration.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
    • 组织特异性Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
    • 疾病相关Ovalocytosis, Southeast Asian
      Spherocytosis 4
      Renal tubular acidosis, distal, autosomal dominant
      Renal tubular acidosis, distal, with hemolytic anemia
      Renal tubular acidosis, distal, with normal red cell morphology
      Cryohydrocytosis
    • 序列相似性Belongs to the anion exchanger (TC 2.A.31) family.
    • 翻译后修饰Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
      Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
      N-glycosylated.
    • 细胞定位Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AE 1 antibody
      • AE1 antibody
      • Anion exchange protein 1 antibody
      • Anion exchanger 1 antibody
      • B3AT_HUMAN antibody
      • Band 3 anion transport protein antibody
      • BND3 antibody
      • CD233 antibody
      • DI antibody
      • Diego blood group antibody
      • EMPB3 antibody
      • EPB3 antibody
      • Erythrocyte membrane protein band 3 antibody
      • Erythroid anion exchange protein antibody
      • FR antibody
      • Froese blood group antibody
      • RTA1A antibody
      • SLC4A1 antibody
      • Solute carrier family 4 anion exchanger member 1 antibody
      • Solute carrier family 4 member 1 antibody
      • SW antibody
      • Swann blood group antibody
      • Waldner blood group antibody
      • WD antibody
      • WD1 antibody
      • WR antibody
      • Wright blood group antibody
      see all

    Anti-Band 3 antibody [EPR1426] 图像

    • Anti-Band 3 antibody [EPR1426] (ab108414) at 1/1000 dilution + Red blood cell lysate at 10 µg

      Predicted band size : 102 kDa
    • ab108414, at 1/100 dilution, staining Band 3 in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • ab108414, at 1/100 dilution, staining Band 3 in paraffin-embedded Human lung tissue by Immunohistochemistry.
    • ab108414 staining Band 3 in Rhesus monkey erythroid cells by Immunocytochemistry/ Immunofluorescence.
      Cells were fixed in methanol and then incubated with ab108414 at a 1/100 dilution for 1 hour. The secondary used was an FITC conjugated goat anti-rabbit polyclonal used at a 1/100 dilution.

      See Abreview

    Anti-Band 3 antibody [EPR1426] (ab108414)参考文献

    ab108414 has not yet been referenced specifically in any publications.

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    Thank you very much for your interest our products. To our knowledge, ab108414 has not been tested in FACS. It looks like none of our anti-Band 3 antibodies have been validated in flow, but I did want to point out that ab108414 does not work in mouse o...

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    Application Immunocytochemistry/ Immunofluorescence
    Sample Rhesus monkey Cell (erythroid cells)
    Specification erythroid cells
    Fixative Methanol
    Permeabilization No
    Username

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    提交于 Jul 17 2012

    Thank you for contacting us. I am sorry for the confusion. Please select according to the following options.

    If the antibody was used in Immunofluorescence on cells then select ICC/IF.

    If on frozen tissue then select IHC-Fr
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    Thank you for your email.

    You need to write the discount code when submitting the Abreview in additional notes box. Please buy the antibody without any hesitation.

    Best of luck for your research.

    DISCOUNT CODE: XXXXXX
    Expiration date: 18-08-2012

    I am very pleased to hear you would like to accept our offer and test ab108414 in Macaca Mulatta. This code will give you: 1 free [PRIMARY ANTIBODY] OR VALUE OFF OF ORDER before the expi...

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    Thank you for accepting my offer.

    Unfortunately you would need the discount code before the purchase. Could you tell me which antibody you would like to buy?

    Thank you for contacting us.

    The macaca Band 3 protein is about 94% homologous to the human protein. It seems that the anti human Band 3 antibodies will cross react with Macaca proteins also.

    You can choose any of the anti Band 3 a...

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    Thank you very much for your phone call.

    We currently do not have Rhesus Monkey Band 3 specific antibodies. The Band 3 antibodies we have are ab11012, ab77236, ab78067, ab104998, ab108414, ab125070 and they seems specific to N-terminal cytopl...

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    Thank you for contacting us.

    The peptide immunogen maps between amino acids 35 and 65 of human Band3 (UniProt: P02730). It is closer to the N-term.

    There is a possibility that ab104998 immunogen might be extracellular but I am wai...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"