BALB/3T3全细胞裂解液(ab7901)

概述

  • 产品名称BALB/3T3全细胞裂解液
  • 常规说明Cell line: BALB/3T3 clone A31 (Mouse embryonic fibroblast).
    Growth media: DMEM and 10% FBS (Fetal bovine serum).


    BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.

  • 经测试应用适用于: WBmore details

性能

  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 5% Beta mercaptoethanol, 1X SDS
  • Concentration information loading...
  • 裂解物说明BALB/3T3 clone A31 cell lysate was prepared by homogenization in modified RIPA buffer(150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • 研究领域

    应用

    Our Abpromise guarantee covers the use of ab7901 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use at an assay dependent dilution. Ready to load; 20 µg/lane is recommended for mini gel.

    BALB/3T3 whole cell lysate (ab7901)参考文献

    ab7901 has not yet been referenced specifically in any publications.

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    Thank you very much for contacting us with your enquiry.

    I'm sorry to hear that there were no bands in Western blot using this antibody. I would expect any of the MEF cell lines to work as a positive control, but I suggest using a whole cell ...

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    Thank you for contacting Abcam and for your interest in our whole cell lysates.

    Regarding these products, they are not tested for protein composition. They are only tested for protein concentration and confirmed to not be degraded.
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